Thermo Fisher Scientific Complement C3 Monoclonal Antibody (11H9)
마우스 C3 단백질을 검출하는 단클론 항체로, Western blot, ELISA, Flow cytometry, IHC 등 다양한 응용에 사용 가능. Rat IgG2a 형식의 비결합형 항체로, PBS와 BSA 함유 완충액에 보관. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:50 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:50 | - |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 1:50 | - |
| ELISA | 1:50 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fungi |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 11H9 |
| Immunogen | C57BL/6 thymocytes saturated with rat anti-Thy-1 monoclonal antibody of IgG2b subclass (RmT1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 0.1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1073809 |
Product Specific Information
- MA1-40046 detects C3 from mouse samples.
- Successfully used in flow cytometry, immunohistochemistry (frozen, paraffin), ELISA, immunofluorescence, immunoprecipitation, and Western blot.
- ELISA: Antibody used as coating 0.5 µg/well in PBS.
- Immunogen: Mouse C3 acute phase protein.
Target Information
C3 is a key component of the complement system where classical and alternative activation pathways converge at the C3 activation step, producing C3a and C3b.
The molecular mass of C3 is approximately 185 kDa and consists of two chains (110 kDa and 75 kDa) linked by disulfide bonds.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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