Thermo Fisher Scientific Complement C3 Monoclonal Antibody (11H9)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:50

-

Immunohistochemistry (Frozen) (IHC (F))

1:50

-

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

1:50

-

ELISA (ELISA)

1:50

-

Product Specifications

Species Reactivity

Mouse

Published species

Fungi

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

11H9

Immunogen

C57BL/6 thymocytes saturated with rat anti-Thy-1 monoclonal antibody of IgG2b subclass (RmT1) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Complement C3,uniProtId:P01027-1,ncbiNodeId:10090,antigenRange:1-1663,antigenLength:1663,antigenImageFileName:MA1-40046_Complement_C3_P01027-1_Rat.svg,antigenImageFileNamePDP:MA1-40046_Complement_C3_P01027-1_Rat_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein G

Storage buffer

PBS with 0.1% BSA

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1073809

Product Specific Information

MA1-40046 detects C3 from mouse samples.

MA1-40046 has been successfully used in flow cytometry, immunohistochemistry (frozen, paraffin), ELISA, immunofluorescence, immunoprecipitation, and Western blot applications.
ELISA: Antibody was used as coating 0.5 µg/well in PBS.

The MA1-40046 immunogen is mouse C3 acute phase protein.

Target Information

C3 is a key component of the complement system since classical and alternative activation pathways merge at the C3 activation step when C3 is split into C3a and C3b. The molecular mass of C3 is 185 kDa and it consists of two chains (110 kDa and 75 kDa) held together by disulfide bonds.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.