Thermo Fisher Scientific Complement C4 Monoclonal Antibody (16D2)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• MA1-40047 detects complement C4 from mouse samples.
• Successfully used in immunohistochemistry (frozen), immunoprecipitation, ELISA, immunofluorescence, and Western blot applications.
• Immunogen: mouse complement factor C4.
• Suggested positive control: T-cell regions of mouse spleen.

Target Information

This gene encodes the acidic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single-chain precursor, which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus and type I diabetes mellitus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have one, two, or three copies of this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.