Thermo Fisher Scientific Complement C4 Monoclonal Antibody (16D2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:50

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:50

-

ELISA (ELISA)

1:50

-

Immunoprecipitation (IP)

1:50

-

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

16D2

Immunogen

Thymocytes decorated with Thy-1 antibody and complement components if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Complement C4,uniProtId:P01029-1,ncbiNodeId:10090,antigenRange:1-1738,antigenLength:1738,antigenImageFileName:MA1-40047_Complement_C4_P01029-1_Rat.svg,antigenImageFileNamePDP:MA1-40047_Complement_C4_P01029-1_Rat_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

100 µg/mL

Purification

Protein G

Storage buffer

PBS with 0.1% BSA

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1073827

Product Specific Information

MA1-40047 detects C4 from mouse samples.

MA1-40047 has been successfully used in immunohistochemistry (frozen), immunoprecipitation, ELISA, immunofluorescence and Western blot applications.

The MA1-40047 immunogen is mouse complement factor C4.

Suggested positive Control: T-cell regions of Mouse spleen.

Target Information

This gene encodes the acidic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus and type I diabetes mellitus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.