Thermo Fisher Scientific Phospho-NFkB p65 (Ser536) Recombinant Rabbit Monoclonal Antibody (NFkBS536-C8)
Recombinant rabbit monoclonal antibody recognizing phospho-NFkB p65 (Ser536). Human reactive, validated for Western blot. Offers high specificity, lot-to-lot consistency, and animal-origin-free formulation. Supplied as liquid, 0.5 mg/mL, stored at -20°C.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–1,000 ng/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | NFkBS536-C8 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Ser536 of human phospho NFkB |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2745052 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the extensive rabbit immune repertoire
Target Information
Nuclear factor kB p65 (NF-kB p65), encoded by the RELA gene on human chromosome 11, is also known as RelA. It belongs to the Rel protein family and forms part of the NF-kB complex with subunits p50 or p52.
Upon TNF-α stimulation, IKK phosphorylates IκB, leading to its degradation and the release of NF-kB. NF-kB p65 plays a crucial role in the transcription of the immunoglobulin k (kappa) gene in mature B-lymphoid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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