Thermo Fisher Scientific Methyl-SOX2 (Lys119) Polyclonal Antibody
SOX2 단백질의 Lys119 메틸화 형태를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot 및 Immunocytochemistry에 적합. Human, Mouse, Rat 반응성. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Methylated-Sox2 (Lys-119) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Lys-119 in mouse Sox2. This site is well conserved in rat and human Sox2, and has high homology with the conserved site in Sox1 (Lys-127) and Sox3 (Lys-145), but low homology to other Sox family members. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2942998 |
Product Specific Information
The antibody was cross-adsorbed to unmethylated Sox2 (Lys-119) peptide before affinity purification using methylated-Sox2 (Lys-119) peptide.
This antibody detects a 34 kDa protein on SDS-PAGE immunoblots of mouse F9 stem cells, as well as human Sox2 recombinant protein methylated by Set7 methyltransferase.
Target Information
SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and cell fate determination.
The SOX2 gene product is required for stem-cell maintenance in the central nervous system and regulates gene expression in the stomach.
SOX2 lies within an intron of the SOX2 overlapping transcript (SOX2OT) gene.
SOX2 protein may act as a transcriptional activator after forming complexes with other proteins.
Mutations in the SOX2 gene are associated with bilateral anophthalmia, optic nerve hypoplasia, and syndromic microphthalmia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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