Thermo Fisher Scientific Methyl-SOX2 (Lys119) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:100

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Methylated-Sox2 (Lys-119) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Lys-119 in mouse Sox2. This site is well conserved in rat and human Sox2, and has high homology with the conserved site in Sox1 (Lys-127) and Sox3 (Lys-145), but low homology to other Sox family members.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2942998

Product Specific Information

The antibody was cross adsorbed to unmethylated Sox2 (Lys-119) peptide before affinity purification using methylated-Sox2 (Lys-119) peptide. This antibody antibody detects a 34 kDa* protein on SDS-PAGE immunoblots of mouse F9 stem cells, as well as human Sox2 recombinant protein that was methylated by Set7 methyltransferase.

Target Information

SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.