Thermo Fisher Scientific LC3B Recombinant Rabbit Monoclonal Antibody (1S3P9)
LC3B 단백질을 인식하는 재조합 토끼 단일클론 항체로, Western blot, IHC, ICC/IF, ELISA, IP 등 다양한 응용에 적합합니다. 인간, 마우스, 랫트 반응성. HEK293 세포 발현, 고순도 친화 크로마토그래피 정제. -20°C 보관, 연구용.
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Thermo Fisher Scientific LC3B Recombinant Rabbit Monoclonal Antibody (1S3P9)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:4,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:200–1:800 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5 µg–4 µg antibody for 200 µg–400 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 1S3P9 |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 2–107 of human LC3B (Q9GZQ8) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.05 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2911863 |
Product Specific Information
- Positive test controls: HeLa, 293T, C6, NIH/3T3
- Target localization: Cytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton
Immunogen sequence:
PSEKTFKQRR TFEQRVEDVR LIREQHPTKI PVIIERYKGE KQLPVLDKTK FLVPDHVNMS ELIKIIRRRL QLNANQAFFL LVNGHSMVSV STPISEVYES EKDEDG
Target Information
LC3B (Autophagy Marker Light Chain 3B, MAP1A/MAP1B LC3 B) is encoded by the gene MAP1LC3B in humans. LC3B is associated with microtubule assembly and plays an important role in neurogenesis and autophagy.
During autophagy, LC3B contributes to the formation of autophagosomes that mediate degradation of cytoplasmic constituents. It is involved in processes related to cancer, aging, metabolic, neurodegenerative, and cardiovascular/pulmonary diseases.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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