Thermo Fisher Scientific NFkB p65 Recombinant Rabbit Monoclonal Antibody (5P4U2)
NFkB p65 단백질을 검출하기 위한 Thermo Fisher Scientific의 재조합 토끼 단클론 항체. Western blot, IHC, ICC, ELISA, ChIP 등 다양한 응용에 적합. 인간, 마우스, 랫드, 비인간 영장류 반응성. 고순도 친화 크로마토그래피 정제 및 안정한 액상 형태.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| ChIP assay (ChIP) | 5 µg antibody for 10–15 µg of chromatin |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 5P4U2 |
| Immunogen | Synthetic peptide corresponding to amino acids 450–551 of human NF-κB p65 (Q04206) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.03 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2911861 |
Product Specific Information
- Positive test controls: MCF7, 293T, Mouse spleen, Mouse liver, HeLa
- Subcellular localization: Cytoplasm, Nucleus
Immunogen sequence:
LGALLGNSTD PAVFTDLASV DNSEFQQLLN QGIPVAPHTT EPMLMEYPEA ITRLVTGAQR PPDPAPAPLG APGLPNGLLS GDEDFSSIAD MDFSALLSQI SS
Target Information
Nuclear factor kB p65 (NF-κB p65) is encoded by the RELA gene located on chromosome 11 in humans. It is also known as RelA and belongs to the Rel family of transcription factors. NF-κB p65 forms a heterodimer with p50 or p52 subunits and plays a critical role in regulating immune and inflammatory responses. Upon TNF-alpha stimulation, IKK phosphorylates IκB, leading to its degradation and allowing NF-κB p65 to translocate to the nucleus, where it activates transcription of target genes such as immunoglobulin kappa in mature B-lymphoid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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