Thermo Fisher Scientific Caspase 8 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2 µg/mL

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

-

Immunocytochemistry (ICC/IF)

2-20 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 16 amino acid synthetic peptide from near the carboxy terminus of human Caspase-8 isoform A. The immunogen is located within the last 50 amino acids of Caspase-8. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Caspase 8,uniProtId:Q14790-1,ncbiNodeId:9606,antigenRange:479,antigenLength:479,antigenImageFileName:PA5-20118_Caspase_8_Q14790-1_Rabbit.svg,antigenImageFileNamePDP:PA5-20118_Caspase_8_Q14790-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11153608

Product Specific Information

This antibody reacts with human Caspase-8 isoform A.

Suggested positive controls are Jurkat cell lysate, human spleen, mouse spleen, or rat spleen tissue lysate.

PA5-20118 can be used with blocking peptide PEP-0236.

Depending on cell lines or tissues used, either full-length or other cleavage products may be observed.

Target Information

Caspase 8 (CASP8) is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases play a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a pro-domain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. Caspase 8 is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of Caspase 8 suggests that it may interact with Fas-interacting protein FADD. Caspase 8 was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Caspase 8 binds to the death effector domain (DED) of FADD through an analogous DED domain present in tandem in the pro-form of the Caspase 8 protein. Activated Caspase 8 then activates other downstream caspases including Caspase 9, thereby committing the cell to undergo apoptosis. In addition, Caspase 8 also reacts with Jurkat cells and Tonsil. Overexpression of Caspase 8 induces apoptosis, which can be blocked by inhibitors specific for the ICE family. Many alternatively spliced transcript variants encoding different isoforms have been described for Caspase 8, however, not all variants have had their full-length sequences determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.