Thermo Fisher Scientific Caspase 8 Polyclonal Antibody
Caspase-8 단백질 검출용 Thermo Fisher Scientific의 Rabbit Polyclonal 항체. Western blot, IHC, ICC/IF에 적합하며 인간, 마우스, 랫트 반응성. 항원 친화 크로마토그래피로 정제된 액상 항체로, 세포 사멸 연구에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–2 µg/mL | View 5 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 2–20 µg/mL | - |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | 16 amino acid synthetic peptide from near the C-terminus of human Caspase-8 isoform A (within the last 50 amino acids). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11153608 |
Product Specific Information
- Reacts with human Caspase-8 isoform A.
- Suggested positive controls: Jurkat cell lysate, human spleen, mouse spleen, rat spleen tissue lysate.
- Compatible blocking peptide: PEP-0236.
- Depending on sample type, full-length or cleavage products may be observed.
Target Information
Caspase 8 (CASP8) is a cysteine-aspartic protease involved in apoptosis execution. It exists as an inactive proenzyme that becomes active through proteolytic processing, forming large and small subunits. Caspase 8 interacts with FADD via its death effector domain, playing a key role in Fas-mediated apoptosis and other apoptotic pathways. It has been implicated in neurodegenerative diseases such as Huntington’s disease. Overexpression induces apoptosis, which can be inhibited by ICE family-specific inhibitors. Multiple alternatively spliced isoforms exist.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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