Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), NovaFluor Blue 585, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV & Violet Series
• eFluor Series
• FITC, PE, PE-Cyanine Series
• PerCP Series
• Super Bright Series
• NovaFluor Blue, Red, UV, Violet, Yellow Series
• Custom conjugation available upon request

Product Specific Information

The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule, a 32–34 kDa cell surface receptor expressed as CD8αβ or CD8αα dimers.

• CD8αβ: Found on thymocytes and αβ TCR T cells
• CD8αα: Found on γδ TCR T cells, intestinal IELs, and dendritic cells

CD8 binds MHC class I and associates with tyrosine kinase p56lck, playing a role in T cell development and activation.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes (≤0.5 µg/test, 100 µL final volume)

Recommendations:

• Titrate antibody for optimal performance
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains (propidium iodide, 7-AAD, DAPI not compatible)
• Add 5 µL of CellBlox Plus Blocking Buffer per 100 µL cell sample (10³–10⁸ cells)

NovaFluor conjugates utilize Phiton™ technology with engineered nucleic acid dye structures for optimized fluorescence and minimal spillover.

Excitation: 494 nm
Emission: 585 nm
Laser: 488 nm (Blue)

Target Information

CD8 (Cluster of Differentiation 8) is a type I transmembrane glycoprotein of the immunoglobulin family.

• Functions as a co-receptor with TCR for MHC class I molecules
• Expressed on T cells, NK cells, monocytes, dendritic cells
• CD8α chain binds MHC-I; forms heterodimers (CD8αβ) or homodimers (CD8αα)
• Activation leads to recruitment of Lck kinase and CTL activation
• Differentiation of CD8+ T cells enhanced by IL-2, IL-12, and TGF-β1

For Research Use Only. Not for use in diagnostic procedures.

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