Thermo Fisher Scientific pan Synuclein Polyclonal Antibody
Rabbit polyclonal antibody recognizing pan Synuclein (alpha, beta, gamma). Validated for WB and IHC-P. Predicted reactivity with rat. Suitable for brain tissue studies. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50 | - |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide near N-terminus of human alpha, beta, gamma synuclein protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2193263 |
Product Specific Information
This antibody is predicted to react with rat samples based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes followed by cooling at room temperature for 20 minutes.
Following antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is brain tissue.
Target Information
Synucleins are small, soluble proteins expressed primarily in neural tissue and in certain tumors.
The family includes three known proteins: alpha-synuclein, beta-synuclein, and gamma-synuclein.
- Alpha- and beta-synuclein proteins are found primarily in brain tissue, mainly in presynaptic terminals.
- Gamma-synuclein protein is found primarily in the peripheral nervous system and retina.
Its expression in breast tumors makes it a candidate marker for tumor progression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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