Thermo Fisher Scientific TGF beta-3 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody is predicted to react with chicken, canine, mouse, porcine, and rat based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes followed by cooling at room temperature for 20 minutes.
Following antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is placenta tissue.

Target Information

Transforming growth factor beta 3 (TGF beta 3) is the third member of the transforming growth factor family of cytokines, which also includes TGF beta 1 and beta 2.
These cytokines are secreted in precursor form consisting of a bioactive C-terminal domain attached to an N-terminal domain known as latency associated protein (LAP).
Cleavage of LAP results in the mature protein, which functions as a disulfide-linked homodimer.
As with all members of the family, TGF beta 3 is highly conserved across species, with mouse and human TGF beta 3 demonstrating 100% sequence homology and cross-species activity.
The members of this family can be expressed by most cell types and exert pleiotropic effects, including suppression of B- and T-cell effector activity, mediation of tissue healing, and suppression of tumor proliferation.
Promotion of CD4+CD25+ T-cell expansion is a newly discovered function of the TGF beta cytokines, indicating an important role in protection against autoimmunity.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.