Thermo Fisher Scientific AFP Monoclonal Antibody (P5B8)
AFP 단백질을 인식하는 마우스 모노클로날 항체로 Western blot, IHC, ICC, ELISA 등에 사용 가능. 인간 시료 반응성. 단백질 A 정제 및 액상 형태로 제공. 2–8°C 보관, 장기 보관 시 -20°C 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | - |
| ELISA | Assay-dependent | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
| Radioimmune Assays (RIA) | Assay-dependent | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | P5B8 |
| Immunogen | Purified AFP from Cord Blood |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M potassium phosphate with 2.5mM KCl, 0.135M NaCl, 1mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 6 months. For long-term storage, store at -20°C. |
| Shipping Conditions | Wet ice |
| RRID | AB_10987005 |
Product Specific Information
MA5-14666 can be used for immunofluorescence analysis of AFP in the endoderm derived from human embryonic stem cells.
By Western blot, MA5-14666 detects endogenous AFP protein in early hepatocyte-like cells derived from human embryonic stem cells.
Product MA514666 is a smaller package size of MIA1305 (formerly sold as a Seradyn product).
Target Information
AFP (Alpha Fetoprotein) is a major plasma protein produced by the yolk sac, intestinal tract, and liver during fetal life.
AFP expression in adults is often associated with hepatoma or teratoma, though hereditary persistence of AFP may occur without pathology.
AFP is considered the fetal counterpart of serum albumin, with both genes located in tandem on chromosome 4.
AFP exists in monomeric, dimeric, and trimeric forms and can bind copper ions, nickel ions, fatty acids, and bilirubin.
Serum AFP levels are used in prenatal screening for neural tube defects and can be elevated in hepatocellular carcinoma, teratoblastoma, colorectal cancer, pancreatic cancer, and germ cell neoplasms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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