Thermo Fisher Scientific ApoA1 Monoclonal Antibody (311)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

Assay-dependent

-

Radioimmune Assays (RIA)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

311

Immunogen

Purified human plasma Apo A-1 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ApoA1,uniProtId:P02647-1,ncbiNodeId:9606,antigenRange:1-267,antigenLength:267,antigenImageFileName:MA5-14668_ApoA1_P02647-1_House_mouse.svg,antigenImageFileNamePDP:MA5-14668_ApoA1_P02647-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Ion-exchange chromatography

Storage buffer

PBS, pH 7.4, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10987487

Product Specific Information

By sandwich ELISA, MA5-14668 can be used as a detection antibody with Product # 710263 or # 701239 as a coating antibody, to generate a matched pair. Using these matched pairs, recombinant human Apo A-1, but not recombinant mouse Apo A-1, was detected. MA5-14668 can be used to detect Apo A-1 from serum samples. To increase sensitivity of sandwich ELISAs with MA5-14668, a biotinylated detection antibody followed by Streptavidin-HRP is recommended.

By Western blot, MA5-14668 detects recombinant human Apo A-1, but not recombinant mouse Apo A-1. MA5-14668 may not be as successful for detecting endogenous Apo A-1 by Western blot.

Product MA514668 is a smaller package size of MIA1402 (formerly sold as a Seradyn product).

Target Information

Apolipoprotein A-1 (apo A-1) in human plasma is a major protein component of high density lipoprotein (HDL). The molecular mass is 24 kDa. Apo A-1 is a cofactor for enzymes involved in the normal turnover of cholesterol and is produced in the liver. Plasma concentration is 270-380 mg/100 mL.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.