Thermo Fisher Scientific 14-3-3 beta Recombinant Rabbit Monoclonal Antibody (SD0837)
14-3-3 beta 단백질을 인식하는 재조합 토끼 모노클론 항체로 높은 특이성과 재현성을 제공. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용 가능. HEK293 세포에서 발현된 동물 유래 성분 없는 포뮬레이션. 단기 4°C, 장기 -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SD0837 |
| Immunogen | Synthetic peptide corresponding to C-terminal Human 14-3-3 alpha + beta aa 197–246 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809697 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences are cloned from immunoreactive rabbits, and individual clones are screened for optimal production.
Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to large rabbit immune repertoire
Target Information
14-3-3 is a family of acidic, soluble proteins conserved from yeast to mammals and expressed in all eukaryotic cells. Seven isoforms (beta, gamma, epsilon, eta, zeta, sigma, tau/theta) are encoded by distinct genes and form homo- and hetero-dimeric cup-shaped structures.
This protein interacts with over 100 binding partners and regulates key proteins involved in:
- Signal transduction
- Cell cycle
- Transcriptional control
- Cell proliferation
- Apoptosis
- Ion channel physiology
14-3-3 proteins often require phosphorylation of serine or threonine residues in target sequences. They are abundantly expressed in the brain and detected in cerebrospinal fluid of patients with neurological disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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