Thermo Fisher Scientific H3K4me3T6ph Polyclonal Antibody
H3K4me3T6ph 변형 히스톤 H3에 특이적인 Thermo Fisher Scientific의 폴리클로날 항체. WB, IHC, ICC, ChIP 등 다양한 응용에 적합. Rabbit IgG 기반, 0.68 mg/mL 농도. 고순도 친화 크로마토그래피 정제. -20°C 보관, 반복 동결·해동 피함.
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Thermo Fisher Scientific H3K4me3T6ph Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (IHC) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| ChIP assay (ChIP) | 2–5 µg/1x10^6 cells |
| Dot blot (DB) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | C. elegans, Chicken, Fruit fly, Human, Mouse, Plant, Rat, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Histone H3 [Trimethyl Lys4, p Thr6] affinity purified antibody prepared from rabbit serum immunized with synthetic trimethylated/phosphorylated peptides surrounding Lysine 4 and Threonine 6 of human Histone H3.2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.68 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 30% glycerol |
| Contains | 0.01% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This antibody reacts with human Histone H3.2.
BLAST analysis suggests cross-reactivity with Human, Mouse, and C. elegans.
Predicted to react with Rat, Chicken, Xenopus, Drosophila, and Plant based on 100% sequence homology.
Cross-reactivity with Histone H3 from other sources has not been determined.
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of eukaryotic chromatin.
Each nucleosome consists of ~146 bp of DNA wrapped around an octamer of core histones (H2A, H2B, H3, H4).
Linker histone H1 interacts with DNA between nucleosomes to form higher-order chromatin structures.
The H3 gene is intronless, lacks polyA tails, and contains a palindromic termination element.
It is found in a histone cluster on chromosome 1, representing the telomeric copy among duplicated histone genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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