
Thermo Fisher Scientific H3K9me2T6ph Polyclonal Antibody
H3K9me2T6ph 변형 히스톤 H3.2를 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. WB, IHC, ICC, ChIP 등 다양한 응용 가능. 고순도 친화 크로마토그래피 정제, 안정적 액상 형태로 -20°C 보관.
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Thermo Fisher Scientific H3K9me2T6ph Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (IHC) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ChIP assay (ChIP) | 2–5 µg/1×10⁶ cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | C. elegans, Chicken, Fruit fly, Human, Mouse, Plant, Rat, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Histone H3 [p Thr6, Dimethyl Lys9] affinity purified antibody prepared from whole rabbit serum produced by repeated immunizations with a synthetic dimethylated/phosphorylated peptide surrounding Lysine 9/Threonine 6 of human Histone H3.2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.49 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This antibody reacts with human Histone H3.2.
BLAST analysis suggests cross-reactivity with Human, Mouse, and C. elegans.
Predicted to react with Rat, Chicken, Xenopus, Drosophila, and Plant based on 100% sequence homology.
Cross-reactivity with Histone H3 from other sources has not been determined.
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fiber in eukaryotes.
This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of H2A, H2B, H3, and H4.
Chromatin fiber is further compacted through interaction with linker histone H1.
This gene is intronless and encodes a member of the histone H3 family, found in a histone cluster on chromosome 1.
Transcripts lack polyA tails and contain a palindromic termination element.
This record represents the telomeric copy of one of four duplicated histone genes in the cluster.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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