Thermo Fisher Scientific RNF20 Monoclonal Antibody (OTI2B9), TrueMAB
RNF20 단백질을 인식하는 Mouse 단클론 항체로 Western blot 및 IHC(P) 실험에 적합. 인간 RNF20 아미노산 493-754 구간을 항원으로 제작. 무보존제, 동결건조 형태로 제공되며 재구성 및 결합 실험에 용이. 연구용으로만 사용.
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Thermo Fisher Scientific RNF20 Monoclonal Antibody (OTI2B9), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2B9 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 493–754 of human RNF20 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional round of desalting using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
RNF20 is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. It selectively suppresses the expression of certain genes, including proto-oncogenes and growth-related genes, by interfering with the chromatin recruitment of transcription elongation factor SII (TFIIS).
RNF20 also positively regulates the p53 tumor suppressor and numerous histone H2A/H2B genes. Together with the deubiquitinase USP44, RNF20 modulates stem cell differentiation through H2B monoubiquitination.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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