Thermo Fisher Scientific Phospho-AMPKa (Ser79) Polyclonal Antibody
AMPKα1 단백질의 Ser79 인산화 부위를 인식하는 Rabbit Polyclonal Antibody. Western blot 및 IHC(P) 실험에 적합. 항원 친화 크로마토그래피로 정제된 액상 시약으로, 세포 내 신호전달 연구에 활용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide surrounding the epitope -CGSPN- with a phosphorylation site at Ser79 of AMPK alpha 1 protein from Carassius auratus origin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | 25 mM Tris with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Serum starved HEK293
- Cellular location: Cytoplasm
Target Information
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the γ phosphate of ATP, onto an acceptor amino acid in a substrate protein.
By this mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating processes such as:
- Cellular metabolism
- Transcription
- Cell cycle progression
- Cytoskeletal rearrangement and movement
- Apoptosis and differentiation
With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. They are classified into eight major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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