Thermo Fisher Scientific Mesothelioma Monoclonal Antibody (ME1)

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

2-5 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2-5 µg/mL

-

Flow Cytometry (Flow)

-

View 1 publication 1 publication

Radioimmune Assays (RIA)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

ME1

Immunogen

Mesothelioma cell line SPC111.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325451

Product Specific Information

MA2-310 detects a surface membrane glycoprotein preferentially expressed on normal and malignant mesothelial cells from human tissues. This antibody does not detect most lung adenocarcinoma cell lines, or well and moderately differentiated pulmonary adenocarcinomas.

MA2-310 has been successfully used in indirect immunofluorescence, immunocytochemistry, immunohistochemistry and solid-phase radioimmunoassay procedures. This antibody does not react in Western blot procedures. Immunohistochemical staining of sarcomatoid mesotheliomas with MA2-310 results in intense staining of mesothelial cells.

It has been reported that this antibody reacts with a 200 kDa surface membrane glycoprotein preferentially expressed on normal and malignant mesothelial cells, but does not react with most lung adenocarcinoma cell lines, or well and moderately differentiated pulmonary adenocarcinomas. In studies, this antibody was shown to react with all mesotheliomas examined, with 50%-99% of cells of both pleural and peritoneal mesotheliomas stained. Literature reports have indicated that the antibody does not bind with many common breast (MCF-7, T47D, ZR75-1, BT20, SJC38), ovarian (OVCA429, OVCA432, OVCA434), colon (SW480, SW620, CX1, WIDR, Co112), and renal-cell (FOHN, ZNH1) carcinoma cell lines, nor with several leukemia (K562, U937, CEM) or fibroblast (WI38, R2F) cell lines. Some reactivity has been reported with some pulmonary tumors of squamous-cell and small-cell carcinoma origin, adenocarcinoma of the breast, and ovary, and gastrointestinal adenocarcinomas. Also, melanomas and poorly differentiated pulmonary adenocarcinoma tumors have reacted with this antibody.

MA2-310 will not react with formalin-fixed, or alcohol-fixed, paraffin embedded tissues. Use of frozen, acetone fixed sections or cell suspensions is recommended.

The MA2-310 antigen is mesothelioma cell line SPC111.

Reconstitute with PBS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.