Thermo Fisher Scientific p300 Monoclonal Antibody (RW109)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:100-1:200

-

Immunoprecipitation (IP)

1:10-1:500

-

ChIP assay (ChIP)

Assay-Dependent

-

Product Specifications

Species Reactivity

Human, Mink, Mouse, Non-human primate, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

RW109

Immunogen

Fusion protein containing residues 1572-2371 of human p300. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:p300,uniProtId:Q09472-1,ncbiNodeId:9606,antigenRange:1572-2371,antigenLength:2414,antigenImageFileName:MA1-16623_p300_Q09472-1_House_mouse.svg,antigenImageFileNamePDP:MA1-16623_p300_Q09472-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.1% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

RRID

AB_568669

Product Specific Information

Suggested positive control: HeLa nuclear extract.

Target Information

EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. p300 is related by sequence to CPB (CREB-binding protein [CREB: cyclic-AMP responsive element binding protein]), and like CPB can stimulate transcription through activation of CREB. This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A. EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.