Thermo Fisher Scientific p300 Monoclonal Antibody (RW105)
p300 단백질을 인식하는 Mouse monoclonal antibody (Clone RW105). Western blot, ICC/IF, Flow cytometry, IP, ChIP 등 다양한 응용에 적합. Human, Mouse, Rat 등 반응. Protein G 정제, 1 mg/mL 농도, PBS buffer에 0.02% sodium azide 포함.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:250–1:1,000 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | View 1 publication |
| Flow Cytometry (Flow) | 1 µg/mL | - |
| Immunoprecipitation (IP) | 1:10–1:500 | - |
| ChIP assay (ChIP) | Assay-Dependent | View 1 publication |
| Immunomicroscopy (IM) | Assay-Dependent | - |
| In vitro Assay (IV) | Assay-Dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mustelid, Mouse, Non-human primate, Rat |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RW105 |
| Immunogen | Fusion protein containing residues 1572–2371 of human KAT3B/p300 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_568668 |
Product Specific Information
- Suggested positive control: HeLa nuclear extract.
Target Information
EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein.
p300 is related by sequence to CBP (CREB-binding protein) and can stimulate transcription through activation of CREB.
This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A.
EP300 also acts as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), playing a role in the stimulation of hypoxia-induced genes such as VEGF.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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