Thermo Fisher Scientific H3K9acS10ph Polyclonal Antibody
H3K9acS10ph 변형 히스톤 H3를 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC/IF에 사용 가능. 다양한 종에 반응하며, NIH3T3 세포에서 검증됨. 액상 형태로 제공되며 4°C 또는 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Amphibian, Bovine, C. elegans, Chicken, Fruit fly, Human, Mouse, Rat, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic, acetylated and phosphorylated (acetyl K9 & phospho S10) peptide, conjugated to KLH, corresponding to the N-terminal residues 7-20 of human Histone H3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 3.7 mg/mL |
| Purification | IgG fraction |
| Storage buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_795753 |
Product Specific Information
- Recommended positive control: NIH3T3 cells treated with sodium butyrate
- PA1-25462 is expected to cross-react with mouse, rat, Xenopus laevis, bovine, C. elegans, chicken, Drosophila melanogaster, and other species due to sequence homology
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Together with four core histone proteins, it forms the nucleosome structure by binding to DNA.
Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
Post-translational modifications of histone H3, such as methylation, acetylation, and phosphorylation, influence chromatin structure and gene expression by altering binding affinity for transcription factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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