Thermo Fisher Scientific JAB1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/ml

-

Immunohistochemistry (Paraffin) (IHC (P))

10-20 µg/mL

-

Immunocytochemistry (ICC/IF)

5 µg/ml

-

Immunoprecipitation (IP)

4-6 µg/mL

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide: SGSGMAQKTWELANNMQ with C-terminal added cysteine, conjugated to KLH, corresponding to amino acids 4-20 of Human JAB1. The corresponding sequence is identical in mouse and rat.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.9 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_794694

Product Specific Information

Recommended positive controls: NIH3T3, PC12, human breast carcinoma.

PA1-25464 is expected to cross react with mouse (100% conserved) and rat (100% conserved) due to sequence homology.

Target Information

JAB-1 is a 38 kDa protein which was originally described as a coactivator for cJUN. It is present in a large protein complex called the Signalosome, which has been proposed to play a signaling role in mammalian cells. This protein complex appears to be analogous to the COP9 complex in plants, which is important in light-mediated plant development. Although the specific function of JAB-1 in this complex is unclear, it has recently been implicated in targeting proteins for degradation by the proteasome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.