Thermo Fisher Scientific IgA Monoclonal Antibody (mA-6E1), FITC, eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 21 publications 21 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

mA-6E1

Conjugate

FITC FITC FITC

View additional formats

• APC
• PE
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465220

Product Specific Information

Description: The mA-6E1 monoclonal antibody reacts with the heavy chain of mouse IgA. It does not react with other isotypes of antibody, including IgD, IgG or IgM, or with other species isotypes. IgA, which is mainly found as a dimer through association of the J chain, is found in mucous secretions, but not in plasma. Fluorochrome conjugated mA-6E1 is used as a detection secondary for mouse IgA primary antibodies.

Applications Reported: This mA-6E1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This mA-6E1 antibody has been tested by flow cytometric analysis as a second step to detect mouse IgA primary antibodies. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Mouse IgA is an immunoglobulin A antibody that is produced by B cells in response to an antigen. It is a crucial component of the mucosal immune system, which protects the body from pathogens that enter through mucosal surfaces such as the respiratory, gastrointestinal, and urogenital tracts. Mouse IgA is composed of two heavy chains and two light chains, and it is the most abundant immunoglobulin in mucosal secretions. It plays a vital role in neutralizing pathogens and preventing their attachment to mucosal surfaces. Mouse IgA is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.