Thermo Fisher Scientific Cytokeratin 18 Monoclonal Antibody (DC-10), FITC

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 8 publications 8 publications

Immunohistochemistry (IHC)

-

View 9 publications 9 publications

Flow Cytometry (Flow)

1-5 µg/mL

View 2 publications 2 publications

ELISA (ELISA)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Non-human primate, Rhesus monkey

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

DC-10

Immunogen

Human breast carcinoma cell line PMC-42. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cytokeratin 18,uniProtId:P05783-1,ncbiNodeId:9606,antigenRange:1-430,antigenLength:430,antigenImageFileName:MA1-10327_Cytokeratin_18_P05783-1_House_mouse.svg,antigenImageFileNamePDP:MA1-10327_Cytokeratin_18_P05783-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

FITC FITC FITC

View additional formats

• Unconjugated
• Biotin
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.1 mg/mL

Purification

Size-exclusion chromatography

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

15mM sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11154144

Product Specific Information

This antibody reacts with cytokeratin 18 (45 kDa). Cytokeratins are members of intermediate filaments subfamily of intracellular proteins, represented in epithelial tissues.

This antibody will not cross-react with porcine, mouse, rat, hamster, bovine, canine or sheep.

Target Information

Cytokeratin 18 (Keratin 18, KRT18, type I cytoskeletal 18) belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. Further, cytokeratin 18 is found primarily in non-squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas, but not in squamous cell carcinomas. Hepatocellular carcinomas express only cytokeratins 8 and 18. Mutations in the cytokeratin 18 gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for the cytokeratin 18 gene. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.