Thermo Fisher Scientific Phospho-AMPK alpha-1,2 (Thr172) Polyclonal Antibody
AMPK α1/α2 Thr172 인산화 상태를 인식하는 rabbit polyclonal antibody로, Western blot, IHC, ICC/IF에 적합합니다. 고순도 액상 형태로 제공되며, PBS/50% glycerol buffer에 보존됩니다. 세포 에너지 대사 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human PRKAA1 (Accession Q13131), corresponding to amino acid residues around phosphorylated Thr172. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2899186 |
Product Specific Information
This antibody detects endogenous levels of AMPK alpha 1 when phosphorylated at Thr183, or AMPK alpha 2 when phosphorylated at Thr172.
Target Information
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit and non-catalytic beta and gamma subunits.
AMPK acts as a key energy-sensing enzyme that monitors cellular energy status. Under metabolic stress, AMPK becomes activated and phosphorylates enzymes such as acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), which are involved in fatty acid and cholesterol biosynthesis.
Mouse studies suggest that this catalytic subunit helps regulate insulin sensitivity and maintain myocardial energy homeostasis during ischemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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