Thermo Fisher Scientific IgM Monoclonal Antibody (II/41), Super Bright 600, eBioscience
마우스 IgM을 특이적으로 인식하는 II/41 클론 단일클론 항체로, Super Bright 600 형광체 결합 제품입니다. Flow cytometry에 최적화되어 있으며, 높은 형광 안정성과 낮은 비특이적 결합을 제공합니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific IgM Monoclonal Antibody (II/41), Super Bright 600, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 5 publications |
| ELISA | - | View 3 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 600, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | II/41 |
| Conjugate | Super Bright™ 600 |
| Excitation / Emission Max | 414 / 601 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2723276 |
Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant Violet 605
- Brilliant Violet 711
- eFluor 450
- eFluor 570
- eFluor 660
- FITC
- NovaFluor Red 710
- NovaFluor Yellow 610
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 645
- Super Bright 780
- Request custom conjugation
Product Specific Information
The II/41 monoclonal antibody reacts with the µ heavy chain of mouse IgM and does not cross-react with other immunoglobulin classes (IgD, IgG, IgA). IgM is expressed intracellularly during early B-cell development and on mature B cells in bone marrow and peripheral tissues.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Mouse bone marrow cells, ≤0.5 µg/test
- Recommended Conditions: Optimize antibody titration for best performance
Fluorochrome Information:
Super Bright 600 is a tandem dye excited by the violet laser (405 nm) and emits at 600 nm. Use a 610/20 bandpass filter. Protect from light to prevent photo-induced oxidation.
Staining Recommendations:
When using multiple Super Bright dyes, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Fixation Compatibility:
Samples can be stored up to 3 days in the dark at 4°C using IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333).
Excitation / Emission / Laser:
405 nm / 600 nm / Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
Mouse IgM is an immunoglobulin M antibody produced by B cells during early immune response. It is the largest serum immunoglobulin, composed of five heavy and five light chains, and plays a key role in complement activation and antigen agglutination. Widely used in ELISA, Western blotting, and immunohistochemistry for research and diagnostic applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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