Thermo Fisher Scientific CD93 (AA4.1) Monoclonal Antibody (AA4.1), Super Bright 600, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.125 µg/test
• Publications: View 3 publications

Product Specifications

Available Formats

• APC
• Biotin
• eFluor 450
• FITC
• PE
• PE-Cyanine7
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 702
• Request custom conjugation

Product Specific Information

The AA4.1 monoclonal antibody reacts with mouse C1qRp, a 130–140 kDa C-type lectin-like type I transmembrane protein. The AA4.1 antigen is expressed on early B cell development stages in bone marrow, and detectable in adult spleen and fetal liver.

In combination with markers such as Thy-1, Sca-1, c-Kit, CD43, and CD24, AA4.1 expression helps segregate lymphoid-committed progenitors (CLP) into primitive and differentiated subsets. Co-expression with surface IgM and CD23 defines transitional B cell subpopulations:

• T1: AA4.1+/CD23-/sIgM^hi
• T2: AA4.1+/CD23+/sIgM^hi
• T3: AA4.1+/CD23+/sIgM^lo

AA4.1 is also expressed in cytoplasmic vesicles of endothelial cells, megakaryoblasts, and platelets. Clones AA4.1 and 493 recognize different epitopes of the same molecule.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse bone marrow cells. Use ≤0.125 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Titrate for optimal performance.

Fluorochrome Information:
Super Bright 600 is a tandem dye excited by the 405 nm violet laser, emitting at 600 nm. Recommended filter: 610/20 bandpass. Use Super Bright Complete Staining Buffer (SB-4401) when using multiple Super Bright conjugates to reduce non-specific interactions.

Handling Notes:

• Light-sensitive; protect from light.
• Fixation: Use IC Fixation Buffer (00-8222) or 1-step Fix/Lyse Solution (00-5333) for up to 3 days at 4°C.
• Excitation: 405 nm; Emission: 600 nm; Laser: Violet

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

The CD93 protein is a type I membrane glycoprotein originally identified as a myeloid cell-specific marker. Although once thought to be a receptor for C1q, it is now understood to mediate intercellular adhesion and apoptotic cell clearance. Its cytoplasmic tail interacts with moesin, linking it to the cytoskeleton and cytoskeletal remodeling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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