Thermo Fisher Scientific MMP13 Monoclonal Antibody (OTI3G1), TrueMAB
Human MMP13 단백질을 인식하는 Mouse 모노클로날 항체로 WB, IHC(P), ICC/IF에 사용 가능. Lyophilized 형태로 제공되며, 1 mg/mL 농도. PBS buffer(8% trehalose)로 보존, -20°C 보관. 세포외기질 단백질 분해 연구용에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3G1 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 104–471 of human MMP13 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional round of desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
MMP13 (collagenase 3) is involved in the degradation of extracellular matrix proteins such as fibrillar collagen, fibronectin, tenascin-C, and aggrecan.
It cleaves triple-helical collagens (types I, II, III) with highest activity toward soluble type II collagen, and can degrade collagen types IV, XIV, and X.
MMP13 is essential for wound healing, tissue remodeling, cartilage degradation, bone development, and ossification.
Mutations in the MMP13 gene are associated with spondyloepimetaphyseal dysplasia Missouri type, metaphyseal anadysplasia 1, and metaphyseal dysplasia Sphar type.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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