Thermo Fisher Scientific E2F1 Monoclonal Antibody (17E2)
E2F1 단백질을 인식하는 Mouse monoclonal antibody (Clone 17E2). Western blot, IHC, ICC/IF, IP 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. Protein G 정제, 1 mg/mL 농도의 액상 형태로 제공. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–5 µg/mL | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | View publications |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | View 1 publication |
| Immunoprecipitation (IP) | Assay-dependent | View 1 publication |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Rat, Human, Mouse |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 17E2 |
| Immunogen | Transactivation/retinoblastoma protein binding domain (a.a. 332–390) of E2F-1 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_558714 |
Product Specific Information
A suggested positive control is E2F-1 transfected Soas2 cells.
Target Information
The E2F1 protein is a member of the E2F family of transcription factors, which play a crucial role in cell cycle control and tumor suppressor protein regulation. E2F1 contains several conserved domains, including:
- DNA-binding domain
- Dimerization domain (interacts with DP proteins)
- Transactivation domain enriched in acidic amino acids
- Tumor suppressor protein association domain (embedded within the transactivation domain)
E2F1, along with E2F2 and E2F3, possesses an additional cyclin-binding domain. It binds preferentially to retinoblastoma protein (pRB) in a cell cycle–dependent manner and can mediate both cell proliferation and p53-dependent or independent apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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