Thermo Fisher Scientific alpha Catenin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 637-906 of human alpha-Catenin (NP_0018942) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:alpha Catenin,uniProtId:P35221-1,ncbiNodeId:9606,antigenRange:637-906,antigenLength:906,antigenImageFileName:PA5-120932_alpha_Catenin_P35221-1_Rabbit.svg,antigenImageFileNamePDP:PA5-120932_alpha_Catenin_P35221-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.36 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2914504

Product Specific Information

Positive test controls include: BT-474, OVCAR3, 293T, MCF7, Mouse lung, Mouse heart, Mouse brain. The target is usually found in the following locations: Cell junction, Cell junction, Cell membrane, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, adherens junction, cytoskeleton.

Immunogen sequence: ELDDSDFETE DFDVRSRTSV QTEDDQLIAG QSARAIMAQL PQEQKAKIAE QVASFQEEKS KLDAEVSKWD DSGNDIIVLA KQMCMIMMEM TDFTRGKGPL KNTSDVISAA KKIAEAGSRM DKLGRTIADH CPDSACKQDL LAYLQRIALY CHQLNICSKV KAEVQNLGGE LVVSGVDSAM SLIQAAKNLM NAVVQTVKAS YVASTKYQKS QGMASLNLPA VSWKMKAPEK KPLVKREKQD ETQTKIKRAS QKKHVNPVQA LSEFKAMDSI

Target Information

Alpha catenin is a member of the b-catenin family of cytosolic proteins. Alpha catenin interacts with N-cadherin and b-catenin on the cytosolic side of adherins junctions to mediate the interaction with the actin cytoskeleton. Alpha catenin is a homodimer in solution, but dissociates to form a 1:1 heterodimer with b-catenin in order to interact with cadherins. It also regulates morphological plasticity of synapses and cerebellar and hippocampal lamination during development. Alpha catenin also functions in the control of the startle modulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.