Thermo Fisher Scientific Biotin Polyclonal Antibody, DyLight 488
DyLight 488 형광 표지를 가진 Biotin 폴리클로날 항체로, WB, ICC/IF, Flow Cytometry, ELISA 등 다양한 응용에 적합. 염소 유래 IgG로 높은 특이성과 감도를 제공. Biotin-KLH 면역원 기반으로 제작되어 연구용으로 최적화됨.
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Thermo Fisher Scientific Biotin Polyclonal Antibody, DyLight 488
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | >1:10,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| Flow Cytometry (Flow) | Assay-dependent |
| ELISA | >1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH) |
| Conjugate | DyLight™ 488 |
| Excitation / Emission Max | 492 / 519 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Biotin conjugated IgG, and Biotin conjugated Bovine Serum Albumin.
Target Information
Biotin is a water-soluble B complex vitamin (Vitamin B7). It is required by all organisms and can only be synthesized by bacteria, yeasts, molds, algae, and some plant species.
Biotin plays a key role in incorporating carbon dioxide into organic compounds and has a molecular weight of approximately 244 Da.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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