Thermo Fisher Scientific SUMO4 Polyclonal Antibody

Applications

Product Specifications

Product Specific Information

The antibody was affinity-purified from rabbit antiserum by affinity chromatography using an epitope-specific immunogen. The purity is >95% (by SDS-PAGE).

Target Information

SUMO4 is a member of the SUMO gene family, which encodes small ubiquitin-related modifiers that covalently modify target lysines in proteins. These modifications regulate subcellular localization, stability, and activity of target proteins. Upon oxidative stress, SUMO4 conjugates to various antioxidant enzymes, chaperones, and stress defense proteins. It can also conjugate to NFKBIA, TFAP2A, and FOS, negatively regulating their transcriptional activity, and to NR3C1, positively regulating its transcriptional activity.

Covalent attachment to SUMO4 substrates requires activation by the E1 complex SAE1–SAE2 and linkage to the E2 enzyme UBE2I. Unlike SUMO1–3, SUMO4 is insensitive to sentrin-specific proteases due to Pro-90, which may impair processing to the mature form. SUMO4 is located in the cytoplasm and specifically modifies IKBA, leading to negative regulation of NF-κB-dependent transcription of the IL12B gene. The M55V substitution has been associated with type I diabetes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.