Thermo Fisher Scientific Phospho-p53 (Ser9) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000

Immunoprecipitation (IP)

• Tested Dilution: 1:100

Product Specifications

Product Specific Information

• It is not recommended to aliquot this antibody.
• This antibody is not cross-reactive with p53 phosphorylated at other sites.

Target Information

The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or pyrimidine nucleotide starvation. p53 regulates transcription of genes such as p21waf1, leading to G1 or G2/M arrest.

Structurally, p53 includes:

• N-terminal transactivation domain
• Central DNA-binding domain
• Oligomerisation domain
• C-terminal regulatory domain

Phosphorylation at Ser15 is crucial for p53 activation and stabilization. Phosphorylation at Ser392 is associated with tumor formation. p53 also plays roles in aging, oxidative stress, and cognitive function. It is expressed at low levels in normal cells but highly in transformed cell lines, contributing to malignancy. Mutations in TP53 cause loss of tumor suppressor activity and are linked to various cancers, including Li-Fraumeni syndrome.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

제품 이미지