Thermo Fisher Scientific CTCF Monoclonal Antibody (G.758.4)
CTCF 단백질을 표적하는 Rabbit Monoclonal 항체로, WB, IHC, ICC, IP, ChIP 등 다양한 응용에 적합합니다. 인간, 마우스, 설치류, 비인간 영장류에 반응하며, 높은 특이성과 재현성을 제공합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:640–1:2560 |
| Immunocytochemistry (ICC/IF) | 1:400–1:1600 |
| Immunoprecipitation (IP) | 1:50 |
| ChIP assay (ChIP) | 10 µl/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | G.758.4 |
| Immunogen | Synthetic peptide corresponding to the carboxy terminus of the human CTCF protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 91 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_10982171 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with BORIS.
Target Information
Transcriptional factor CTCF is a ubiquitously expressed, 11-zinc finger domain-containing, DNA-binding nuclear phosphoprotein involved in multiple aspects of normal gene regulation, including transcriptional repression and activation, gene silencing, chromatin insulation, and regulation of imprinted sites.
CTCF interacts with the CCCTC motif on DNA using different combinations of ZF domains and can bind to HAT- and HDAC-containing complexes, influencing transcriptional activation or repression.
It plays roles in transactivation of the APPB gene, silencing of c-myc, insulation of human beta-globin and DMI myotonic dystrophy locus, and imprinting control of IGF2 and H19 regions.
CTCF also regulates transcription of Pax6 and IRAK2 promoters, suggesting therapeutic potential.
Mutations in CTCF have been associated with breast, prostate, and Wilms’ tumors.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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