Thermo Fisher Scientific PCAF Monoclonal Antibody (E.107.0)
PCAF 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체로, Western blot, IP, ChIP에 적합. 인간, 마우스, 원숭이, 랫트 반응성. 고순도 친화 크로마토그래피 정제, 비교적 높은 농도(358 µg/mL), -20°C 보관. GCN5L2 단백질과 교차 반응 없음.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunoprecipitation (IP) | 1:100 | - |
| ChIP assay (ChIP) | 1:25 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | E.107.0 |
| Immunogen | Synthetic peptide corresponding to the amino terminus of human PCAF protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 358 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_10978155 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with the related GCN5L2 protein.
Target Information
PCAF (histone acetyltransferase KAT2B) functions as a histone acetyltransferase (HAT) to promote transcriptional activation. It has significant histone acetyltransferase activity with core histones (H3 and H4) and nucleosome core particles.
PCAF also acetylates non-histone proteins such as ACLY, PLK4, and TBX5. It inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A.
Additionally, PCAF acts as a circadian transcriptional coactivator enhancing the activity of NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers. It is involved in heart and limb development by mediating acetylation of TBX5 and regulating nucleocytoplasmic shuttling of TBX5.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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