
Thermo Fisher Scientific GNL3 Polyclonal Antibody
Rabbit polyclonal antibody recognizing human GNL3 (Nucleostemin) for WB, IHC, and IP applications. Recommended dilutions range from 1:100 to 1:10,000. Supplied as liquid, 0.2 mg/mL, stored at 4°C. Predicted reactivity with several primate species.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 1–4 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 1 and 50 of human Guanine Nucleotide Binding Protein-Like 3 (Nucleostemin) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Horse, Orangutan, Monkey, Gorilla, and Chimpanzee
Target Information
Arylsulfatase F (ARSF) is a 590-amino acid secretory protein belonging to the sulfatase family of bone and cartilage matrix proteins. It uses calcium as a cofactor to catalyze reactions important for maintaining proper bone composition. Unlike other family members such as Arylsulfatase E, its activity is not inhibited by warfarin. The ARSF gene maps to human chromosome X, which spans approximately 153 million base pairs and contains over 1,000 genes. Chromosome X, together with chromosome Y, determines sex and is associated with conditions such as Turner’s syndrome, color blindness, hemophilia, and Duchenne muscular dystrophy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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