Thermo Fisher Scientific FASN Recombinant Rabbit Monoclonal Antibody (4U9S3)
FASN 단백질을 표적하는 Thermo Fisher Scientific의 재조합 토끼 단일클론 항체(4U9S3). Western blot, IHC, ICC/IF, ELISA, IP에 최적화. 인간 및 생쥐 반응성, 높은 특이성과 재현성 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:800 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5 µg–4 µg antibody for 200 µg–400 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 4U9S3 |
| Immunogen | Synthetic peptide corresponding to amino acids 2400–2500 of human FASN (UniProt ID: P49327) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.64 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2911818 |
Product Specific Information
- Positive test controls: A-549, HeLa
- Target localization: Cytoplasm, Melanosome
- Immunogen sequence:
AVDLIIKSHQ GLDRQELSFA ARSFYYKLRA AEQYTPKAKY HGNVMLLRAK TGGAYGEDLG ADYNLSQVCD GKVSVHVIEG DHRTLLEGSG LESIISIIHS S
Target Information
Fatty acid synthase (FASN) is a multifunctional enzyme that catalyzes the synthesis of palmitate from acetyl-CoA and malonyl-CoA in the presence of NADPH, producing long-chain saturated fatty acids. In certain cancer cell lines, FASN has been observed to fuse with estrogen receptor-alpha (ER-alpha), forming a chimeric protein with the N-terminus of FAS and the C-terminus of ER-alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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