Thermo Fisher Scientific MHC Class II Monoclonal Antibody (OX17), PerCP-eFluor 710, eBioscience

Thermo Fisher Scientific MHC Class II Monoclonal Antibody (OX17), PerCP-eFluor 710, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.25 µg/test
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Product Specifications

Product Specific Information

This OX17 monoclonal antibody reacts with the I-E molecule of rat MHC Class II (RT1-D).
MHC Class II is expressed on antigen-presenting cells such as B cells, dendritic cells, and macrophages.

• Blocking Activity: OX17 antibody has been reported to possess blocking activity.
• Crossblocking: OX17 and HIS19 recognize different epitopes.
• Applications Reported: Suitable for flow cytometric analysis.
• Applications Tested: Tested on rat splenocytes; use ≤ 0.25 µg per test (100 µL final volume).
  • Cell number: 10⁵–10⁸ cells/test (empirical determination recommended).
  • Antibody titration recommended for optimal performance.

Fluorophore Information:
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm).

• Recommended filter: 710/50 bandpass (alternative: 695/40 bandpass).
• Ensure instrument compatibility with this fluorochrome.

Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222-49)
or 1-step Fix/Lyse Solution (Product #00-5333-54)
for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.
Clone-specific performance should be verified empirically.

Excitation: 488 nm
Emission: 710 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

MHC Class II molecules are transmembrane glycoproteins expressed on antigen-presenting cells (macrophages, dendritic cells, B cells).
They bind exogenous antigens and present them to T cells.
The antigen-binding groove between MHC class II alpha and beta chains is open at both ends and accommodates peptides 15–24 amino acids long.

HLA and MHC antibodies are critical tools in Immunopeptidomics, aiding identification and characterization of neoantigens via LC-MS/MS.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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