Thermo Fisher Scientific Cytokeratin 3 Monoclonal Antibody (AE5)
Cytokeratin 3 단백질을 특이적으로 인식하는 단클론 항체로, 인간, 토끼, 소 시료에 적용 가능. IHC, ICC/IF, Flow Cytometry 등 다양한 실험에 사용. Protein G로 정제된 액상 형태로 제공되며, 단기 4°C, 장기 -20°C 보관 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | - |
| Flow Cytometry (Flow) | Assay-dependent | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Human, Rabbit |
| Published Species | Human, Pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AE5 |
| Immunogen | Rabbit corneal epithelial keratins from P3 x 63 Ag8 cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1075197 |
Additional Formats
Product Specific Information
MA1-5763 detects the 64k basic keratin K3 in human, rabbit, rat, and bovine samples.
It has been successfully used in Western blot, immunocytochemistry/immunofluorescence, and immunohistochemistry (paraffin) applications.
The immunogen is rabbit corneal epithelial keratins.
Target Information
Cytokeratin 3 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues.
This type II cytokeratin is specifically expressed in the corneal epithelium with family member KRT12. Mutations in these genes have been associated with Meesmann’s Corneal Dystrophy.
The type II cytokeratins are clustered in a region of chromosome 12q12–q13. (NCBI Gene description)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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