Thermo Fisher Scientific CENPE Monoclonal Antibody (1H12)
Thermo Fisher Scientific의 CENPE Monoclonal Antibody (1H12)는 인간 CENP-E 단백질을 인식하는 마우스 단클론 항체로, Western blot, ICC/IF, Flow Cytometry, IP에 적합합니다. 1 mg/mL 농도의 액상 제품이며, 단백질 정제 후 PBS에 0.1% sodium azide를 포함합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–1 µg/mL | View 1 publication |
| Immunocytochemistry (ICC/IF) | Assay-dependent | - |
| Flow Cytometry (Flow) | 1.0 µg/1×10⁶ cells | - |
| Immunoprecipitation (IP) | 3 µg/300 mg lysate | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1H12 |
| Immunogen | Recombinant full-length CENP-E protein (human) from SP2/0 cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2078916 |
Product Specific Information
This antibody targets a protein with a predicted molecular weight of 312 kDa. It is suitable for Western blot analysis only if immunoprecipitation (IP) is performed first.
Target Information
14-3-3 sigma (also known as stratifin, SFN, epithelial cell marker protein 1) belongs to a conserved family of 14-3-3 proteins found in all eukaryotes. There are seven known mammalian isoforms (epsilon, beta, zeta, gamma, theta, tau, sigma) that regulate numerous biological processes by modulating localization and stability of signaling molecules.
14-3-3 sigma was initially identified as a mammary epithelium marker and later recognized as a key regulator of cell cycle checkpoints. It has been implicated as a prognostic marker in pancreatic cancer and is the only isoform induced by p53 in response to DNA damage. Acting as a p53-regulated inhibitor of G2/M progression, it functions as a tumor suppressor gene that can be inactivated by methylation in epithelial tumors. Two isoforms of human 14-3-3 sigma are produced via alternative splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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