Thermo Fisher Scientific CENPE Monoclonal Antibody (1H12)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Flow Cytometry (Flow)

1.0 µg/1x10^6 cells

-

Immunoprecipitation (IP)

3 µg/300 mg lysate

-

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG, kappa

Class

Monoclonal

Type

Antibody

Clone

1H12

Immunogen

Recombinant full length CENP-E protein (human) from SP2/0 cells if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CENPE,uniProtId:Q02224-1,ncbiNodeId:9606,antigenRange:1-2701,antigenLength:2701,antigenImageFileName:MA1-5758_CENPE_Q02224-1_House_mouse.svg,antigenImageFileNamePDP:MA1-5758_CENPE_Q02224-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Ion-exchange chromatography

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2078916

Product Specific Information

This target has a predicted molecular weight of 312 kDa and is only suitable for Western blot if IP is performed first.

Target Information

14-3-3 sigma (stratifin, SFN, epithelial cell marker protein 1) is a member of a highly conserved family of 14-3-3 proteins that are present in all eukaryotic organisms. There are 7 known mammalian 14-3-3 isoforms epsilon, beta, zeta, gamma, theta, tau and they play important roles in many biological activities by binding to and altering the subcellular localization and/or stability of key molecules in various signaling cascades. 14-3-3 sigma was originally characterized as a human mammary epithelium marker and later rediscovered as an important molecule for cell cycle checkpoint regulation. Recently it has been reported that 14-3-3 sigma may serve as a prognosis marker predicting survival of pancreatic cancer patient treatment. 14-3-3 sigmais the only 14-3-3 isoform induced by the tumor suppressor protein p53, in response to g-irradiation and other DNA-damaging agents. 14-3-3 sigmais a p53-regulated inhibitor of G2/M progression and acts as a tumor suppressor gene that is inactivated by methylation of its 5` CpG islands in epithelial tumor cells. Two isoforms of human 14-3-3 sigma are produced by alternative splicing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.