Thermo Fisher Scientific CD157 Monoclonal Antibody (eBioSY11B5 (SY11B5)), Super Bright 436, eBioscience

Applications

Tested Application

• Flow Cytometry (Flow)

Recommended Dilution

• 5 µL (0.25 µg)/test

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Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• APC
• NovaFluor Blue 660-120S
• NovaFluor Red 710
• NovaFluor Yellow 590
• NovaFluor Yellow 610
• PE
• PE-Cyanine7
• Super Bright 600
• Request custom conjugation

Product Specific Information

The eBioSY11B5 monoclonal antibody recognizes human CD157 (Mo5, BST-1). CD157 is a 42–45 kDa GPI-anchored protein structurally and functionally similar to CD38. Initially identified on monocytes and macrophages, it was later found to be identical to BST-1, expressed on bone marrow stromal cells and capable of stimulating pre-B cell proliferation.

CD157 acts as both enzyme and receptor, involved in NAD⁺ metabolism and regulation of intracellular Ca²⁺ levels. Upon ligand binding, it triggers phosphorylation of cytoplasmic proteins such as focal adhesion kinase (FAK). The detailed mechanism of CD157-mediated signaling remains under investigation.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested on normal human peripheral blood cells at 5 µL (0.25 µg) per test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test.

Super Bright 436 is excited by the violet laser (405 nm) and emits at 436 nm. Use a 450/50 bandpass filter or equivalent. Ensure instrument compatibility.

When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

Bone marrow stromal cell antigen 1 (BST1) is a GPI-anchored molecule facilitating pre-B cell growth. Also known as ADP-ribosyl cyclase 2 and DAMP-1, it is encoded by the BST1 gene in humans. Expression is increased in bone marrow stromal cells from rheumatoid arthritis patients, potentially contributing to B cell abnormalities associated with the disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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