Thermo Fisher Scientific beta Actin Polyclonal Antibody
β-Actin 단백질을 인식하는 Thermo Fisher Scientific의 rabbit polyclonal 항체로, Western blot, IHC, ICC, IP 등 다양한 응용에 사용 가능. 인간, 마우스, 랫 등 다양한 종에 반응하며, 세포 골격 연구 및 로딩 컨트롤용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
| Immunoprecipitation (IP) | 1:100–1:1,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Fruit fly, Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human beta Actin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.26 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792434 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (100%), Rat (100%), Japanese Medaka (98%), Xenopus laevis (99%), Dog (100%), Rabbit (99%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%), Guinea pig (100%).
- Positive Control: 293T, A431, HeLa, HepG2, Neuro2A, CD830, NIH-3T3, Raw264.7, C2C12, mouse liver, rat liver, PC-12, Rat2, drosophila.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Beta actin is one of six actin isoforms identified in humans. It is a non-muscle cytoskeletal protein present in all cell types and plays key roles in cell motility, structure, and integrity.
Beta and gamma actins coexist in most cell types as components of the cytoskeleton. Polymerization of globular actin (G-actin) forms filamentous actin (F-actin), a two-stranded helical structure.
Due to its ubiquitous expression, beta actin is frequently used as a loading control in protein detection assays such as Western blotting. Antibodies against beta actin are valuable tools for studying intracellular distribution and cytoskeletal dynamics.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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