Thermo Fisher Scientific NCF4 Monoclonal Antibody (OTI4E5), TrueMAB

Applications

• Western Blot (WB): 1:2,000 dilution tested

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

The NCF4 gene encodes a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system essential for host defense.
This protein is mainly expressed in myeloid lineage cells and interacts with NCF2 (p67-phox) and NCF1 (p47-phox) to form a complex that activates flavocytochrome b upon cell stimulation.
The PX domain binds phospholipid products of PI(3) kinase, implicating it in PI(3) kinase-mediated signaling.
Phosphorylation of NCF4 negatively regulates enzyme activity.
Alternative splicing results in distinct isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.