Thermo Fisher Scientific NCF4 Monoclonal Antibody (OTI4E5), TrueMAB
NCF4 단백질을 인식하는 Mouse monoclonal antibody로 Western blot에 적합. Human, Mouse, Rat 시료에 반응. Lyophilized 형태로 제공되며 PBS buffer에 trehalose 포함. 연구용으로 세포 신호전달 및 산화효소 복합체 연구에 활용 가능.
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Applications
- Western Blot (WB): 1:2,000 dilution tested
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4E5 |
| Immunogen | Full length human recombinant protein of human NCF4 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The NCF4 gene encodes a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system essential for host defense.
This protein is mainly expressed in myeloid lineage cells and interacts with NCF2 (p67-phox) and NCF1 (p47-phox) to form a complex that activates flavocytochrome b upon cell stimulation.
The PX domain binds phospholipid products of PI(3) kinase, implicating it in PI(3) kinase-mediated signaling.
Phosphorylation of NCF4 negatively regulates enzyme activity.
Alternative splicing results in distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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