Thermo Fisher Scientific CD83 Monoclonal Antibody (Michel-17 (Michel17)), eFluor 660, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.25 µg/test
• Publications: View 3 publications

Product Specifications

Available Formats

• Unconjugated
• Biotin
• FITC
• PE
• Request custom conjugation

Product Specific Information

The Michel-17 monoclonal antibody reacts with mouse CD83, a 45 kDa cell surface glycoprotein belonging to the Ig superfamily. CD83 is expressed predominantly on mature dendritic cells and activated lymphocytes. Cross-linking of CD83 with Michel-17 does not induce activation signals. CD83 plays an important role in T cell development through interaction with its ligand, which is mainly expressed by B220+ cells in the mouse spleen.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on stimulated mouse splenocytes at ≤0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
  Antibody titration is recommended for optimal performance.

Fluorophore Information:
eFluor® 660 replaces Alexa Fluor® 647, emits at 659 nm, and is excited by the red laser (633 nm).
Ensure instrument compatibility.

• Excitation: 633–647 nm
• Emission: 668 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD83 is a 40–45 kDa glycoprotein expressed on peripheral blood dendritic cells. Peripheral lymphocytes can express low levels after stimulation with agents such as Con A or PHA. In immunohistology, CD83 is strongly expressed by interdigitating reticulum cells and weakly by cells in germinal centers. It is also found on Langerhans cells in the skin.

CD83 is a 186-amino-acid single-chain glycoprotein, a member of the immunoglobulin superfamily, with an extracellular V-type Ig-like domain, transmembrane region, and short cytoplasmic tail. It undergoes extensive glycosylation, resulting in a molecular weight about twice the predicted core protein size. CD83+ cells coexpress high levels of MHC class II and various lineage markers (CD2, CD5, CD40, CD78, CD13, CD33, CD36).

Associated Diseases: Plague, Rift Valley Fever

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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