Thermo Fisher Scientific CD23 Monoclonal Antibody (B3B4), NovaFluor Red 710, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.4 µg/test

Product Specifications

Available Formats

• Unconjugated
• Biotin
• Brilliant UV 805
• eFluor 450
• eFluor 660
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 725
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Custom conjugation available

Product Specific Information

Description:
The B3B4 monoclonal antibody reacts with mouse CD23, a 45 kDa type II transmembrane glycoprotein expressed on resting conventional B cells. Its expression changes upon B-cell activation, while B-1 lineage (CD5+ B cells) does not express CD23. CD23 acts as a low-affinity receptor for IgE, involved in IgE regulation and B-cell activation. CD21 is known to bind CD23.

Applications Reported:
Suitable for flow cytometric analysis.

Applications Tested:
Tested by flow cytometry using mouse splenocytes. Recommended ≤0.4 µg per test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best results.

Each product includes one vial of NovaFluor conjugate and one vial of CellBlox Plus Blocking Buffer.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) must be used for optimal staining.
  • Add 5 µL per sample (regardless of number of NovaFluors).
  • For single-color controls: add 5 µL per 100 µL of cell sample (10³–10⁸ cells).

Technology:
NovaFluor conjugates utilize Phiton™ technology based on novel nucleic acid dye structures engineered for optimal spectral properties with reduced spillover and spread.

Excitation/Emission: 639 nm / 710 nm
Laser: 633–640 nm (Red)

Target Information

CD23 is a 45 kDa glycoprotein present on a subset of peripheral blood and tonsil B cells, strongly expressed on EBV-transformed B lymphoblasts. It functions as the low-affinity IgE receptor on B cells and is involved in B-cell growth, differentiation, and IgE regulation. Soluble CD23 acts as a mitogenic factor. Dysregulation is associated with conditions such as chronic conjunctivitis and chronic lymphocytic leukemia.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

제품 이미지