Thermo Fisher Scientific CD11a (LFA-1alpha) Monoclonal Antibody (M17/4), NovaFluor Yellow 755, eBioscience

Applications

• Flow Cytometry (Flow): ≤ 0.4 µg/test

Product Specifications

Additional Available Formats

• Unconjugated
• APC
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 700
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The M17/4 monoclonal antibody reacts with mouse CD11a, the 180 kDa integrin alpha L (LFA-1 alpha chain). LFA-1, formed by association of CD11a with CD18, functions as an adhesion molecule in lymphocyte and granulocyte activity. Ligands include CD54 (ICAM-1), CD102 (ICAM-2), and CD50 (ICAM-3). CD11a is expressed by all leukocytes.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported

• Suitable for flow cytometric analysis.

Applications Tested

• Tested on mouse splenocytes.
• Recommended use: ≤ 0.4 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
• Optimize antibody titration for best results.

Important Notes

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) must be used for optimal staining with NovaFluor conjugates.
  • Add 5 µL per sample (regardless of number of NovaFluors).
  • For single-color controls: 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor Technology

NovaFluor conjugates are based on Phiton™ technology using nucleic acid dye structures for engineered fluorescence with reduced spillover and spread.
Excitation: 552 nm; Emission: 690 nm; Laser: 561 nm (Yellow Laser)

Note: NovaFluor Yellow 755 may non-specifically stain B cells in SJL mice. No non-specific staining observed in BALB/c or C57BL/6 strains.

Target Information

CD11a (LFA-1 alpha, ITGAL) forms the LFA-1 complex with CD18 (αLβ2 integrin). It mediates leukocyte adhesion via ICAM-1, -2, and -3, and participates in T-cell costimulatory signaling. CD11a is expressed on leukocyte membranes and shifts from low- to high-affinity conformation upon stimulation, enabling strong adhesion.
CD11a is involved in immune cell interactions, tissue localization, differentiation, and proliferation control.
Blocking CD11a is a therapeutic target for inflammatory diseases (e.g., Efalizumab/Raptiva).
Two transcript variants encoding different isoforms have been identified.
Associated diseases include benign cephalic histiocytosis and leukocyte adhesion deficiency.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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