Thermo Fisher Scientific CD28 Monoclonal Antibody (37.51)
Mouse CD28 costimulatory receptor를 인식하는 Syrian hamster 모노클로날 항체. IHC, ICC/IF, Flow cytometry, IP, Functional assay 등에 사용 가능. 1 mg/mL 농도의 액상 형태로 제공되며, preservative-free PBS buffer에 저장. T 세포 활성화 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | 1–4 µg/mL | - |
| Flow Cytometry (Flow) | 6 µg/mL | 1 publication |
| Immunoprecipitation (IP) | 1–2 µg/mL | - |
| Functional Assay (Functional) | Assay-dependent | - |
| Blocking Assay (BLOCK) | - | 9 publications |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Syrian hamster / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 37.51 |
| Immunogen | Mouse T cell lymphoma EL-4 cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11155411 |
Product Specific Information
This antibody reacts with the extracellular domain of mouse CD28 costimulatory receptor, a 45 kDa homodimeric protein expressed by thymocytes, mature T cells, and NK cells.
Endotoxin level is less than 0.01 EU/µg of protein (LAL test).
Functional Application: In vitro and in vivo T cell costimulation.
Target Information
CD28 antigen is a 44 kDa disulfide-linked homodimeric T cell surface glycoprotein belonging to the immunoglobulin superfamily. It is constitutively expressed on most peripheral blood T lymphocytes and acts as a critical costimulatory receptor by binding CD80/CD86 on antigen-presenting cells.
CD28 prevents T cells from entering an anergic or apoptotic state and is expressed on all CD4+ and CD8+ T cells in murine peripheral lymphoid organs and blood.
In the thymus, expression is highest on immature CD3-, CD8+, and CD4+8+ cells.
Diseases associated with CD28 dysfunction include mycosis fungoides and Sezary’s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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