Thermo Fisher Scientific T-bet Monoclonal Antibody (eBio4B10 (4B10)), PerCP-Cyanine5.5, eBioscience

Applications and Tested Dilution

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Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 660
• APC
• eFluor 450
• eFluor 660
• PE
• PE-Cyanine5
• PE-Cyanine7
• Request custom conjugation

Product Specific Information

The eBio4B10 monoclonal antibody reacts with mouse and human T-bet, a Th1-specific T-box transcription factor critical to the development of the Th1 CD4⁺ T cell lineage.
T-bet expression is induced by IFN-γ and regulates IFN-γ, IL-12Rβ, and IL-2 expression. It also plays a role in class-switch recombination in B cells.

Applications Reported:
For use in intracellular staining followed by flow cytometric analysis.

Applications Tested:
Tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells.
Recommended ≤ 0.25 µg/test in 100 µL final volume (10⁵–10⁸ cells/test).
Optimize titration for best results.

Recommended Buffer Set:
Use the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00) and follow BestProtocols® Protocol B.

Fluorescence Properties:

• Excitation: 488 nm
• Emission: 695 nm
• Laser: Blue Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

T-bet (TBX21) is a T-cell specific transcription factor containing a T-box DNA-binding domain.
It regulates expression of the Th1 cytokine IFN-γ and contributes to Th1 lineage commitment.
T-bet cooperates with Stat4 in programming chromatin architecture for Th1 gene expression.
Genetic variations are associated with susceptibility to aspirin-induced asthma and nasal polyps.

For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.

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