Thermo Fisher Scientific T-bet Monoclonal Antibody (eBio4B10 (4B10)), PerCP-Cyanine5.5, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 66 publications 66 publications

Product Specifications

Species Reactivity

Human, Mouse, Rhesus monkey

Published species

Human, Mouse, Pig, Rat, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-Cyanine5.5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio4B10 (4B10)

Conjugate

PerCP-Cyanine5.5 PerCP-Cyanine5.5 PerCP-Cyanine5.5

View additional formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 660
• APC
• eFluor 450
• eFluor 660
• PE
• PE-Cyanine5
• PE-Cyanine7
• Request custom conjugation

Excitation/Emission Max

489/679 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_953658

Product Specific Information

Description: The eBio4B10 monoclonal antibody reacts with mouse and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage. T-Bet expression is induced by the Th1 cytokine IFN gamma, and T-Bet also regulates the expression of IFN gamma, likely, at least in part, through the modification of DNA accessibility and histone remodeling. In addition to IFN gamma, T-Bet is also known to regulate the expression of IL-12R beta and IL-2. Moreover, T-Bet plays a role in class-switch recombination in B-cells.

Applications Reported: This eBio4B10 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This eBio4B10 antibody has been tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells. This antibody can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

For intracellular staining with the eBio4B10 monoclonal antibody, it is recommended to use the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to BestProtocols®; Protocol B: One step protocol for intracellular (nuclear) proteins).

Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

T-bet, also known as TBX21, is a T-cell specific transcription factor containing a single T-box DNA-binding domain. It controls the expression of the TH1 cytokine, interferon-gamma. Both Th1 and Th17 cells are crucial in immune regulation and autoimmune disease development. T-bet initiates Th1 lineage development from naive Th precursor cells and directs T cell differentiation to Th1 versus Th17. T-bet is cooperating with Stat4 (signal tranducer and activator of transcription 4) in programming chromatin architecture for Th1 gene expression. Genetic variations in T-bet are associated with susceptibility to aspirin-induced asthma and nasal polyps.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.