Thermo Fisher Scientific Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human GSK3A(Accession P49840), corresponding to amino acid residues around phosphorylated Tyr279. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GSK3 alpha/beta,uniProtId:P49840-1,ncbiNodeId:9606,antigenRange:279,antigenLength:483,antigenImageFileName:PA5-104917_GSK3_alphabeta_P49840-1_Rabbit.svg,antigenImageFileNamePDP:PA5-104917_GSK3_alphabeta_P49840-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:GSK3 alpha/beta,uniProtId:P49841-1,ncbiNodeId:9606,antigenRange:216|279,antigenLength:420,antigenImageFileName:PA5-104917_GSK3_alphabeta_P49841-1_Rabbit.svg,antigenImageFileNamePDP:PA5-104917_GSK3_alphabeta_P49841-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2816390

Product Specific Information

Antibody detects endogenous levels of GSK3 alpha/ beta only when phosphorylated at Tyrosine 279/216.

Target Information

Glycogen synthase kinase-3 (GSK3) is a protein kinase that was originally identified as a regulator of glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of the substrate only occurs if a phosphoserine or phosphotyrosine is present - four residues C-terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, GSK3 alpha and GSK3 beta, and they are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3alpha) by protein kinase B (PKB) causes its inactivation and is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor-1 (HSF-1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimers disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.