Thermo Fisher Scientific PEP12 Recombinant Rabbit Monoclonal Antibody (7H24L3)
Recombinant rabbit monoclonal antibody targeting yeast Syntaxin (Pep12). Validated for Western blot. Offers high specificity, lot-to-lot consistency, and animal origin-free formulation. Suitable for research use only.
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Applications
Western Blot (WB)
- Tested Dilution: 0.1–1 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Yeast |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 7H24L3 |
| Immunogen | Protein corresponding to amino acids 1–268 of yeast Syntaxin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2532339 |
Product Specific Information
This antibody is predicted to react with yeast based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generates a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Syntaxins are a family of SNARE proteins. Yeast (Saccharomyces cerevisiae) has about seven syntaxin-like genes, while humans have fifteen.
Syntaxins interact with proteins such as NSF and SNAP to mediate vesicular transport in exocytic and endocytic pathways, ensuring specificity in vesicle fusion processes.
In yeast, the syntaxin protein is known as Pep12, which functions in vesicle transport from the Golgi apparatus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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